An in vitro evaluation of tyrosine derived polyarylates and polycarbonate as polymeric scaffold for human mesenchymal stem cells osteogenic differentiation
Department of Biomedical Engineering
Master of Science
Arinzeh, Treena Livingston
Hunter, William Corson
Human mesenchymal stem cells
Human mesenchymal stem cells (hMSCs) obtained from the adult bone marrow are multipotential cells that are capable of differentiating along several lineages. They are readily available and abundant source of cells in the field of tissue engineering. For their use in treating certain connective tissue defect or disorders, their success depends at minimum on the use of scaffolds that support differentiation. Therefore , this thesis details a study that systematically evaluated hMSC differentiation on materials that exhibit gradual range in surface properties as a first study for selecting materials as potential scaffolds. Polycarbonates and polyarylates have combinatorial libraries that exhibit a range of surface chemistries leading to changes in the surface properties such as wettability, rigidity and protein adsorption. Structural modifications of their pendant chain and backbone provide a means to modify their physicomechanical, chemical and biological properties. Polymers were coated onto 96-well cell culture plates using the solvent casting technique. The human MSCs isolated from the bone marrow were seeded at a fixed density of 3 x 1 0^4 cells/cm2 in 96-well plates containing coated polymers. The cells were grown for 16 days in the presence or absence of osteogenic supplement. Alkaline phosphatase activity, cell number by DNA quantification and the amount of calcium in the extracellular matrix were measured at day 4,8,12 and 16. In addition, osteocalcin assay was performed at day 16. The results indicated that cell proliferation was highest on more hydrophilic surface; however the osteogenic differentiation was greater on more hydrophobic polymeric substratum.
njit-etd2004-051 (73 pages ~ 6,257 KB pdf)
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Created November 8, 2004