| Title: | An algorithm for estimating the quality of microarrays |
| Author: | |
| Document Type: | Thesis |
| Department: | Federated Biological Sciences Department of NJIT and Rutgers-Newark |
| Degree: | Master of Science |
| Major: | Computational Biology |
| Advisory Committee: |
Recce, Michael
Hart, Ronald Philip
Tolias, Peter P.
|
| Thesis Date: | 2002, August |
| Keywords: |
Microarrays
Microarray evaluation
Molecular biology
|
| Availability: | Unrestricted |
| Abstract: |
Microarray technology is
currently one of the most valuable gene expression tools in molecular
biology allowing the experimenter to simultaneously quantify the expression
of thousands of genes. It is also one of the most difficult tools to use
accurately as each microarray produces a large amount of information that
needs to be inspected and normalized before analysis. As the size of a
microarray or number of replicates increase, the use of manual inspection
becomes impractical. The aim of this thesis is to introduce an algorithm
that evaluates each feature of a microarray from the scanned data file.
A quality score is calculated from various spot parameters, the quality-quotient,
and can be used to automatically assess the quality of the spot. This
quality-quotient can then be utilized to automatically select quality
spots or act as a weighting factor for comparing spots from replicate
microarrays.
|
| Complete Thesis: | njit-etd2002-039
(46 pages ~ 2,284 KB pdf)
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Created May 15, 2003
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