The image of leukocyte adhesion to microvascular walls in the isolated rat perfused heart model has been observed and processed. Some of the fluorescent labeled leukocytes are barely visible due to microscope focal plane and the motion of objects . The purpose of this thesis is to extract leukocyte from the image of the experimental data using image enhancement and analysis (segmentation) techniques and develop a program to handle automatically counting of leukocytes in a predefined scene.