NJIT eTD::njit-etd1988-020::Lakhwala, Fayaz::"Bead design for biodegradation of 2-chlorophenol using microorganisms entrapped in alginate gel"

The performance of immobilized cell reactors varies with a number of parameters, one of which is proper bead design. A mixed microbial population from a waste water treatment plant was immobilized in calcium alginate gel. The viability of these organisms was studied in a microassay reactor by varying parameters such as concentration of calcium chloride, concentration of sodium alginate, temperature of operation, biomass concentration within the beads and concentration of a model compound (2-chlorophenol). The effect of storage on viability over a period of three months was also investigated. It was found possible to have access to active biomass by drying the beads and storing at 4°C.

In addition, polyvinyl alcohol gel and diatomaceous earth beads were also tried as supports for microbe immobilization. Rates of removal of the substrate (2-chlorophenol) were followed in an air sparged semibatch reactor of volume 300 ml, at 37°C. It was found that 40 percent of the removal was by stripping and about 60 percent was due to biooxidation.

Title:	Bead design for biodegradation of 2-chlorophenol using microorganisms entrapped in alginate gel
Author:	Lakhwala, Fayaz
Document Type:	Thesis
Department:	Department of Chemical Engineering, Chemistry and Environmental Science
Degree:	Master of Science
Major:	Chemical Engineering
Advisory Committee:	Sofer, Samir S. Lewandowski, Gordon Trattner, Richard B.
Thesis Date:	1988
Keywords:	Chlorophenols--Biodegradation. Colloids.
Availability:	Unrestricted
Abstract:	The performance of immobilized cell reactors varies with a number of parameters, one of which is proper bead design. A mixed microbial population from a waste water treatment plant was immobilized in calcium alginate gel. The viability of these organisms was studied in a microassay reactor by varying parameters such as concentration of calcium chloride, concentration of sodium alginate, temperature of operation, biomass concentration within the beads and concentration of a model compound (2-chlorophenol). The effect of storage on viability over a period of three months was also investigated. It was found possible to have access to active biomass by drying the beads and storing at 4°C. In addition, polyvinyl alcohol gel and diatomaceous earth beads were also tried as supports for microbe immobilization. Rates of removal of the substrate (2-chlorophenol) were followed in an air sparged semibatch reactor of volume 300 ml, at 37°C. It was found that 40 percent of the removal was by stripping and about 60 percent was due to biooxidation.
Complete Thesis:	njit-etd1988-020 (65 pages ~ 2,075 KB pdf)
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