Bead design for biodegradation of 2-chlorophenol using microorganisms entrapped in alginate gel
Department of Chemical Engineering, Chemistry and Environmental Science
Master of Science
Sofer, Samir S.
Trattner, Richard B.
The performance of immobilized cell reactors varies with a number of parameters, one of which is proper bead design. A mixed microbial population from a waste water treatment plant was immobilized in calcium alginate gel. The viability of these organisms was studied in a microassay reactor by varying parameters such as concentration of calcium chloride, concentration of sodium alginate, temperature of operation, biomass concentration within the beads and concentration of a model compound (2-chlorophenol). The effect of storage on viability over a period of three months was also investigated. It was found possible to have access to active biomass by drying the beads and storing at 4°C.
In addition, polyvinyl alcohol gel and diatomaceous earth beads were also tried as supports for microbe immobilization. Rates of removal of the substrate (2-chlorophenol) were followed in an air sparged semibatch reactor of volume 300 ml, at 37°C. It was found that 40 percent of the removal was by stripping and about 60 percent was due to biooxidation.
njit-etd1988-020 (65 pages ~ 2,075 KB pdf)
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Created September 30, 2012